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75 µg of protein lysates were heated for 10 min at 95 °C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 4–20% Criterion TGX pre-cast gels (Bio-Rad #5671093)

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If left untreated, a B12 deficiency can lead to more severe health issues, including neurological damage

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